RESEARCH ARTICLE


An Assessment of the Microbial Diversity Present on the Surface of Naturally Incubated House Wren Eggs



Beth A. Potter*, Brian M. Carlson, Andrea E. Adams, Margaret A. Voss, J.-L. Vasseur
Department of Biology, The Behrend College, Pennsylvania State University, Erie, PA, 16563, USA


© 2013 Potter et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Penn State Erie, The Behrend College, 4205 College Drive, Erie, PA 16563, USA; Tel: 814-898-6510; Fax: 814-898-6213; E-mail: bap16@psu.edu


Abstract

During ovipositioning, avian eggshells become susceptible to bacterial and fungal growth and studies have shown that a community of these microorganisms, or microflora, is maintained on eggshells throughout the incubation process. To determine the possible role of these microorganisms on embryonic development, it is first important to understand the composition of the microbial community present on the surface of the egg. A limited amount of studies have been published in this area; thus, the objective of this study was to broaden this area of study and determine what bacterial communities are found on the surface of naturally-incubated House Wren eggs across three stages of incubation (pre, early, and late) as defined by egg temperature. Our data uniquely suggest that the eggshell microflora is dynamic and that this may be regulated by temperature fluctuations due to intermittent incubation behavior. Using culture-based techniques, 46 different bacterial species were identified belonging to 13 bacterial families and 20 genera. The majority of bacteria belonged to the Pseudomonas, Staphylococcus, Stenotrophomonas, or Burkholderia genera and have been previously associated with avian eggs and nests. Bacteria within the Pseudomonas genus were the most predominant and we hypothesize that their maintenance may be linked to their ability to produce antibiotic substances called bacteriocins. The bacterial composition of the microflora isolated in this study also suggests that avian egg microfloras are derived from environmental origins.

Keywords: Avian incubation, bacteria, eggshell, House Wren, Pseudomonas, temperature.