RESEARCH ARTICLE


Season-Related Differences in the Biosynthetic Activity of the Neonatal Chicken Pineal Gland



Aneta Piesiewicz*, Podobas Ewa, Kędzierska Urszula, Joachimiak Ewa, Markowska Magdalena, Majewski Pawel, Skwarlo-Sonta Krystyna
Department of Animal Physiology, Faculty of Biology, University of Warsaw, Poland


© 2010 Piesiewicz et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to the Department of Animal Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland; Tel: +48 22 55 41 025; Fax: +48 22 55 41 030; E-mails: Mnechaeva2003@yahoo.com


Abstract

Influence of the season of hatch on the functional characteristics of the pineal gland was examined in neonatal Hi-Line male chickens. The pineal glands from 2-day-old birds hatched in summer and winter, and kept from the day of hatch in artificial lighting conditions (12L:12D), were isolated under dim red light in the middle of the day or night. The pineal glands were analyzed to characterize their melatonin biosynthetic activity: (1) expression of the Arylalkylamine– N–acetyltransferase and Hydroxyindole-O-methyltransferase genes, encoding the final two enzymes of the melatonin biosynthesis pathway; (2) the activity of AA-NAT and HIOMT; and (3) the content of the main substrates of this pathway, tryptophan (TRY) and serotonin (5-HT). Daily changes in pineal AA-NAT activity were observed in chickens hatched in both seasons, with a more pronounced nocturnal increase in summer. In contrast, the level of Aa-nat gene expression, although exhibited the same nocturnal/diurnal pattern in both seasons, was much lower in the summer. The activity of HIOMT was season- and daytime-independent. In “winter” chickens the pineal content of 5-HT was low and stable, while in “summer” birds it was correlated with levels of AA-NAT activity and Aa-nat gene expression. TRY content was very high and exhibited neither daily nor seasonal changes. The pineal gland of newly hatched chickens kept in controlled 12L:12D conditions exhibits daily variations in melatonin biosynthetic activity influenced by the season. This suggests a maternal effect on the perinatal/postnatal development of the circadian clock residing in the chicken pineal gland.

Keywords: Melatonin biosynthesis pathway, neonatal chicken, pineal gland, seasonal changes.